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Image Search Results
Journal:
Article Title: Severe respiratory syncytial virus infections and reduced interferon-? generation in vitro
doi: 10.1111/j.1365-2249.2004.02582.x
Figure Lengend Snippet: RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) and interleukin-4 (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
Article Snippet: The permeabilized cells were incubated with fluoroscein isothiocyanate (FITC)-labelled antibodies against T cell-receptor (TCR)-Vβ2 chain (Beckman Coulter, Unterschleissheim, Germany), and phycoerythrin (PE)-labelled anti-interferon-γ or
Techniques: Derivative Assay, Infection, Cell Culture, Expressing, Flow Cytometry