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Becton Dickinson phycoerythrin (pe)-labelled anti-interferon-γ or anti-il-4
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Becton Dickinson pe-conjugated anti-rat il-4
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Becton Dickinson anti-il-4 mab
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Becton Dickinson anti-mouse interleukin-4 (il-4) mab
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Becton Dickinson anti–il-4 abs
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Abfrontier ltd rabbit anti-rat il-1b
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
Rabbit Anti Rat Il 1b, supplied by Abfrontier ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems goat anti il 1b
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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ImmunoKontact bvd6 (anti-il-4) abs
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
Bvd6 (Anti Il 4) Abs, supplied by ImmunoKontact, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio il 1b
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Boster Bio interleukin 4
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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Schering-Plough corporation il-4
RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) <t>and</t> <t>interleukin-4</t> (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.
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RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) and interleukin-4 (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.

Journal:

Article Title: Severe respiratory syncytial virus infections and reduced interferon-? generation in vitro

doi: 10.1111/j.1365-2249.2004.02582.x

Figure Lengend Snippet: RSV modulated cytokine production in patients with a history of RSV bronchiolitis and controls. Blood samples were obtained at 1 year of age. Monocyte derived dendritic cells were infected with RSV (MOI 10) and cultured with peripheral blood naive T cells and TSST 1 for 3 days. The expression of interferon-γ (a) and interleukin-4 (b) in TSST 1 reactive Vβ2+ cells was measured by flow cytometry as described in Methods. The infants were grouped according to their history of RSV infection. Horizontal lines denote the medians. Significant differences between the groups are indicated.

Article Snippet: The permeabilized cells were incubated with fluoroscein isothiocyanate (FITC)-labelled antibodies against T cell-receptor (TCR)-Vβ2 chain (Beckman Coulter, Unterschleissheim, Germany), and phycoerythrin (PE)-labelled anti-interferon-γ or anti-IL-4 (Pharmingen, Heidelberg, Germany) for 20 min at 4°C (Medac, Hamburg, Germany).

Techniques: Derivative Assay, Infection, Cell Culture, Expressing, Flow Cytometry